Real-time quantitative PCR (RQ-PCR) is a standardized tool for Minimal Residual Disease (MRD) monitoring in pediatric patients with Acute Lymphoblastic Leukemia (ALL), showing a prognostic impact also in the field of Hematopoietic Stem Cell Transplantation (HSCT). This approach can be limited by the availability of diagnostic material. Recently, droplet digital PCR (ddPCR) was introduced and has already led to several research breakthroughs in various fields. The main advantage of this method is that there is no need for external references, since it allows for absolute quantification of target DNA. We evaluated the applicability of ddPCR for MRD analysis in pediatric ALL transplanted patients and we compared the prognostic impact of MRD assessed by RQ-PCR and ddPCR before HSCT.

Comparison of MRD detection by RQ-PCR and droplet digital PCRin pediatric Acute Lymphoblastic Leukemia before Hematopoietic Stem Cell Transplantation

Mimma Campeggio;Luca Trentin;Sheila Camnasio;Emanuela Giarin;Barbara Buldini;Elisa Carraro;Marta Pillon;Giuseppe Basso;Federica Lovisa
2017

Abstract

Real-time quantitative PCR (RQ-PCR) is a standardized tool for Minimal Residual Disease (MRD) monitoring in pediatric patients with Acute Lymphoblastic Leukemia (ALL), showing a prognostic impact also in the field of Hematopoietic Stem Cell Transplantation (HSCT). This approach can be limited by the availability of diagnostic material. Recently, droplet digital PCR (ddPCR) was introduced and has already led to several research breakthroughs in various fields. The main advantage of this method is that there is no need for external references, since it allows for absolute quantification of target DNA. We evaluated the applicability of ddPCR for MRD analysis in pediatric ALL transplanted patients and we compared the prognostic impact of MRD assessed by RQ-PCR and ddPCR before HSCT.
Abstract Book
978-90-825958-1-9
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/3256293
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact