Modeling the oxygen inhibition in microalgae: An experimental approach based on photorespirometry

Microalgae cultivation has been the object of relevant interest for many industrial applications. Where high purity of the biomass/product is required, closed photobioreactors (PBRs) appear to be the best technological solution. However, as well as cost, the major drawback of closed systems is oxygen accumulation, which is well known to be responsible for growth inhibition. Only a few quantitative approaches have attempted to describe and model oxygen inhibition, which is the result of different biological mechanisms. Here, we have applied a photorespirometric protocol to assess and quantify the effect of high oxygen concentration on photosynthetic production rate. In particular, the effects of light intensity and biomass concentration were assessed, resulting in different maximum inhibitory oxygen concentrations. Literature models available were found not to fully represent experimental data as a function of concentration and light. Accordingly, a new formulation was proposed and validated to describe the photosynthetic rate as a function of external oxygen concentration.