Aims To examine the effects of nociceptin (NC) and endomorphin 1 (EM1) on electrical ®eld stimulation (EFS)-induced contractions of the human vas deferens (hVD). Methods Concentration-response curves to NC and EM1 were constructed in the absence and in presence of peptidase inhibitors (PI). In some experiments a NC receptor antagonist, [Phe1y(CH2-NH)Gly2]NC(1±13)NH2 [F/G], 10 mM) or naloxone (1 mM) were included. Results All data are mean(95%CI). In the presence of PI, NC inhibited twitches (Emax=67(44,90)%; pEC50=7.28(6.95,7.61)). NC inhibition was sensitive to [F/G]. EM1 also inhibited twitches both in the absence (Emax=82(73,91)% pEC50=7.07 (6.92,7.22)) and presence (Emax=83(76,90)%; pEC50=7.00(6.91, 7.09)) of PI. EM1 inhibition was sensitive to naloxone. Conclusions These data suggest that hVD express NC and opioid receptors that inhibit neurogenic contractions.
Effects of nociceptin and endomorphin 1 on the electrically stimulated human vas deferens
CALO G;
2001
Abstract
Aims To examine the effects of nociceptin (NC) and endomorphin 1 (EM1) on electrical ®eld stimulation (EFS)-induced contractions of the human vas deferens (hVD). Methods Concentration-response curves to NC and EM1 were constructed in the absence and in presence of peptidase inhibitors (PI). In some experiments a NC receptor antagonist, [Phe1y(CH2-NH)Gly2]NC(1±13)NH2 [F/G], 10 mM) or naloxone (1 mM) were included. Results All data are mean(95%CI). In the presence of PI, NC inhibited twitches (Emax=67(44,90)%; pEC50=7.28(6.95,7.61)). NC inhibition was sensitive to [F/G]. EM1 also inhibited twitches both in the absence (Emax=82(73,91)% pEC50=7.07 (6.92,7.22)) and presence (Emax=83(76,90)%; pEC50=7.00(6.91, 7.09)) of PI. EM1 inhibition was sensitive to naloxone. Conclusions These data suggest that hVD express NC and opioid receptors that inhibit neurogenic contractions.Pubblicazioni consigliate
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