Female C57BL/6J mice, nine weeks old, were fed diets differing in the amount and type of fat (5% and 23.5% w/w olive oil and corn oil), for a period of two weeks. The levels of total and individual polyamines putrescine, spermidine and spermine) were measured in the mammary glands after two weeks of experimental diet. For this analysis we used a liquid-liquid chromatography apparatus equipped with a fluorescence detector. The levels of putrescine and spermine were higher in the 23.5% fat diets compared to the 5% fat diets. This increase was induced by olive and corn oil, while the content of spermidine increased only in high olive oil diets. Mice in estrus had higher polyamine levels in mammary tissue than mice in diestrus on the same diet. The results were analyzed with a regression analysis model considering the interaction of variables like estrus, diestrus, type of polyamine and type of dietary fat. The increase in mammary polyamine levels induced by high dietary fats is an indicator of increased proliferation since cell polyamines increase during the early phase of cell growth both in normal and in cancer mammary tissues. Although the role of dietary fat in mammary carcinogenesis has not been well established yet, our data demonstrate that a variation of cell proliferation induced by fat may modify the response to chemical carcinogens.

High dietary fat increases polyamine content of murine mammary glands

LASAGNI, LAURA;BIGGERI, ANNIBALE;
1990

Abstract

Female C57BL/6J mice, nine weeks old, were fed diets differing in the amount and type of fat (5% and 23.5% w/w olive oil and corn oil), for a period of two weeks. The levels of total and individual polyamines putrescine, spermidine and spermine) were measured in the mammary glands after two weeks of experimental diet. For this analysis we used a liquid-liquid chromatography apparatus equipped with a fluorescence detector. The levels of putrescine and spermine were higher in the 23.5% fat diets compared to the 5% fat diets. This increase was induced by olive and corn oil, while the content of spermidine increased only in high olive oil diets. Mice in estrus had higher polyamine levels in mammary tissue than mice in diestrus on the same diet. The results were analyzed with a regression analysis model considering the interaction of variables like estrus, diestrus, type of polyamine and type of dietary fat. The increase in mammary polyamine levels induced by high dietary fats is an indicator of increased proliferation since cell polyamines increase during the early phase of cell growth both in normal and in cancer mammary tissues. Although the role of dietary fat in mammary carcinogenesis has not been well established yet, our data demonstrate that a variation of cell proliferation induced by fat may modify the response to chemical carcinogens.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/3409019
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