Few data are available on Campylobacter spp. presence in chickens in Ethiopia. Due to its importance for both the poultry sector and public health, a sampling activity was planned to evaluate Campylobacter spp. presence in layer farms in Bishoftu and Mojo, Central Ethiopia. Twenty cloacal pooled samples were collected and tested with molecular assays for detection and Sanger-sequenced for species identification. As a secondary aim, samples were also tested for Salmonella spp. by PCR, and all samples were negative. On the other hand, 70% of cloacal swab pools were positive for Campylobacter spp.: 71.4% of the positive samples belonged to C. jejuni species, 21.4% to C. avium and 7.1% to C. helveticus. Campylobacter spp. was identified in almost all farms regardless of farm and flock size, age and hybrid types of the birds and antimicrobial treatment. Campylobacter jejuni is a common finding in chickens, whereas species such as C. avium and C. helveticus were newly reported in Ethiopia, revealing a variability that needs to be monitored in light of the public health significance of this pathogen.

Molecular Survey and Identification of Campylobacter spp. in Layer Farms in Central Ethiopia

Behailu Assefa Wayou
Formal Analysis
;
Gezahegne Mamo Kassa
Membro del Collaboration Group
;
Alessandra Mondin
Data Curation
;
Claudia Maria Tucciarone
Conceptualization
;
Mattia Cecchinato
Supervision
;
Daniela Pasotto
Writing – Review & Editing
2022

Abstract

Few data are available on Campylobacter spp. presence in chickens in Ethiopia. Due to its importance for both the poultry sector and public health, a sampling activity was planned to evaluate Campylobacter spp. presence in layer farms in Bishoftu and Mojo, Central Ethiopia. Twenty cloacal pooled samples were collected and tested with molecular assays for detection and Sanger-sequenced for species identification. As a secondary aim, samples were also tested for Salmonella spp. by PCR, and all samples were negative. On the other hand, 70% of cloacal swab pools were positive for Campylobacter spp.: 71.4% of the positive samples belonged to C. jejuni species, 21.4% to C. avium and 7.1% to C. helveticus. Campylobacter spp. was identified in almost all farms regardless of farm and flock size, age and hybrid types of the birds and antimicrobial treatment. Campylobacter jejuni is a common finding in chickens, whereas species such as C. avium and C. helveticus were newly reported in Ethiopia, revealing a variability that needs to be monitored in light of the public health significance of this pathogen.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/3421274
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