TIPIZZAZIONE DI CEPPI DI CAMPYLOBACTER JEJUNI: CONFRONTO TRA DUE PROTOCOLLI RFLP

The cheese (ham) skipper, Piophila casei (Linnaeus), is a member of the "skipper fly" family (Piophilidae) and it is detritivore feeding on decaying matter. Larvae are typically found on high-protein substrates such as cheese, ham and carcasses. Life cycle is strictly influenced by temperature and food. In this work, we tested development time and larval length of P. casei on ham (Prosciutto di Parma) at five constant different temperatures and we calculated ADD (Accumulated Degree Days) and Isomegalen diagram in order to estimate the infestation time of this product. The minimum development temperature obtained by extrapolation of the development rates measured among 28°C and 20°C was 10.7 °C, and the ADD 28°C (egg-pupa) resulted to be 129.8. Finally, the larvae developed considerably faster on the ham than those fed with the standard diet.