The effects which parental cell density at the time of fusion with polyethylene glycol has on the production of viable somatic hybrids, frequency of polykaryocytes and nuclear multiplicity have been determined for Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Hybrid frequencies appeared to be highest where polykaryons with more than two nuclei prevailed over dikaryons, suggesting that a substantial fraction of viable hybrids is derived from fusion events which involve more than two cells. © 1981.
Evidence for the derivation of mammalian somatic hybrids from polykaryocytes.
LANFRANCHI, GEROLAMO;
1981
Abstract
The effects which parental cell density at the time of fusion with polyethylene glycol has on the production of viable somatic hybrids, frequency of polykaryocytes and nuclear multiplicity have been determined for Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Hybrid frequencies appeared to be highest where polykaryons with more than two nuclei prevailed over dikaryons, suggesting that a substantial fraction of viable hybrids is derived from fusion events which involve more than two cells. © 1981.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
