The chain-breaking of bovine spongiform encephalopathy (BSE) spread between ruminant species and from bovine to humans has been performed by the ban on ruminant-derived proteins in ruminant feeds. The necessity to evaluate the fraudolent use of rendered protein supplements leads to new and more sensitive analytical methods. Bovine mithocondrial DNA seems to be a good marker for this purpose and the polymerase chain reaction leads to an in vitro amplification of a specific target DNA sequence. In this study we could detect up to 0.0625% of bovine-derived material scattered in a commercial feedstuff. The DNA extraction method used is effective also on different kinds of foods wich have been subjected to high temperature (canned meat) or long ripening time such as Parmesan cheese.

Identificazione di specie animale in farine per uso zootecnico ed in prodotti sottoposti a trattamento termico

MALOCCO, CAMILLA;NOVELLI, ENRICO;
2000

Abstract

The chain-breaking of bovine spongiform encephalopathy (BSE) spread between ruminant species and from bovine to humans has been performed by the ban on ruminant-derived proteins in ruminant feeds. The necessity to evaluate the fraudolent use of rendered protein supplements leads to new and more sensitive analytical methods. Bovine mithocondrial DNA seems to be a good marker for this purpose and the polymerase chain reaction leads to an in vitro amplification of a specific target DNA sequence. In this study we could detect up to 0.0625% of bovine-derived material scattered in a commercial feedstuff. The DNA extraction method used is effective also on different kinds of foods wich have been subjected to high temperature (canned meat) or long ripening time such as Parmesan cheese.
Atti della Società Italiana delle Scienze Veterinarie
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/1359695
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