Plasmid-minus derivatives of Rhizobium leguminosarum biovar trifolii have been isolated. Cured strains lacked symbiotic properties, however they showed increased heavy metal resistance. In the presence of 70 ppm chromium the parent strain, unlike cured derivatives, is unable to grow explanta but can nevertheless nodulate clover. We propose that rhizobia can circumvent exposure to the heavy metal by entering the plant roots. Acetylene reduction tests showed that nodulated plants, grown in the presence of 10 ppm of chromium, had an increased nitrogenase activity compared to the control plants.
Effects of cadmium, chromium and copper on symbiotic and free-living Rhizobium leguminosarum biovar trifolii.
CASELLA, SERGIO;SQUARTINI, ANDREA
1988
Abstract
Plasmid-minus derivatives of Rhizobium leguminosarum biovar trifolii have been isolated. Cured strains lacked symbiotic properties, however they showed increased heavy metal resistance. In the presence of 70 ppm chromium the parent strain, unlike cured derivatives, is unable to grow explanta but can nevertheless nodulate clover. We propose that rhizobia can circumvent exposure to the heavy metal by entering the plant roots. Acetylene reduction tests showed that nodulated plants, grown in the presence of 10 ppm of chromium, had an increased nitrogenase activity compared to the control plants.File in questo prodotto:
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