The invention relates to luminescent chimeric proteins comprising a first N-terminal protein sequence, a second protein sequence and a third C-terminal protein sequence wherein: (i) said first and said third protein sequence are a leader sequence and an anchor sequence belonging to at least a receptor localized on a plasma membrane site; (ii) said second protein sequence encodes for the full-length or partial sequence of a photoprotein and is inserted in frame between said first and said third sequence (i); said chimeric protein being addressed to said plasma membrane site of the cell wherein it is expressed.
CHIMERIC PROTEINS FOR MEASURING ATP CONCENTRATIONS IN PERICELLULAR SPACE AND RELATED SCREENING METHOD
RIZZUTO, ROSARIO
2005
Abstract
The invention relates to luminescent chimeric proteins comprising a first N-terminal protein sequence, a second protein sequence and a third C-terminal protein sequence wherein: (i) said first and said third protein sequence are a leader sequence and an anchor sequence belonging to at least a receptor localized on a plasma membrane site; (ii) said second protein sequence encodes for the full-length or partial sequence of a photoprotein and is inserted in frame between said first and said third sequence (i); said chimeric protein being addressed to said plasma membrane site of the cell wherein it is expressed.File in questo prodotto:
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