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EnglishThe dynamic interactions of the main pathways for active Ca2+ transport have been analysed in living cells by altering the expression of their components. The plasma membrane (PMCA) and the endoplasmic reticulum (ER) (SERCA) Ca2+ pumps were transiently overexpressed in CHO cells, and the Ca2+ homeostasis in the subcellular compartments was investigated using specifically targeted chimaeras of the Ca2+-sensitive photoprotein aequorin, In resting cells, overexpression of the PMCA and SERCA pumps caused a reduction and an increase in ER [Ca2+] levels, respectively; while no significant differences were detected in cytosolic and mitochondrial [Ca2+]. Upon stimulation with an inositol 1,4,5-trisphosphate (IP3)-generating agonist, the amplitude of the mitochondrial and cytosolic Ca2+ rises correlated,vith the ER [Ca2+] only up to a threshold value, above which the feedback inhibition of the IP3 channel by Ca2+ appeared to be limiting.
| Titolo: | Effects of PMCA and SERCA pumps overexpression on the kinetics of cell Ca2+ signalling |
| Autori: | |
| Data di pubblicazione: | 2000 |
| Rivista: | |
| Abstract: | The dynamic interactions of the main pathways for active Ca2+ transport have been analysed in living cells by altering the expression of their components. The plasma membrane (PMCA) and the endoplasmic reticulum (ER) (SERCA) Ca2+ pumps were transiently overexpressed in CHO cells, and the Ca2+ homeostasis in the subcellular compartments was investigated using specifically targeted chimaeras of the Ca2+-sensitive photoprotein aequorin, In resting cells, overexpression of the PMCA and SERCA pumps caused a reduction and an increase in ER [Ca2+] levels, respectively; while no significant differences were detected in cytosolic and mitochondrial [Ca2+]. Upon stimulation with an inositol 1,4,5-trisphosphate (IP3)-generating agonist, the amplitude of the mitochondrial and cytosolic Ca2+ rises correlated,vith the ER [Ca2+] only up to a threshold value, above which the feedback inhibition of the IP3 channel by Ca2+ appeared to be limiting. |
| Handle: | http://hdl.handle.net/11577/2463658 |
| Appare nelle tipologie: | 01.01 - Articolo in rivista |
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