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EnglishAequorin is a Ca(2+) sensitive photoprotein suitable to measure intracellular Ca(2+) transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca(2+) uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca(2+) levels and mitochondrial Ca(2+) uptake in mammalian cells.
| Titolo: | Ca(2+) Measurements in Mammalian Cells with Aequorin-based Probes | |
| Autori: | ||
| Data di pubblicazione: | 2017 | |
| Rivista: | ||
| Abstract: | Aequorin is a Ca(2+) sensitive photoprotein suitable to measure intracellular Ca(2+) transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca(2+) uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca(2+) levels and mitochondrial Ca(2+) uptake in mammalian cells. | |
| Handle: | http://hdl.handle.net/11577/3228900 | |
| Appare nelle tipologie: | 01.01 - Articolo in rivista |
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