Detection of apoptosis-inducing factor in involuting mammary tissue by immunoelectron microscopy

Immunoelectron microscopy was used to study the subcellular localisation of apoptosis-inducing factor (AIF) in involuting bovine mammary tissue. AIF was detected using a polyclonal antibody and secondary anti-rabbit antibody conjugated to colloidal gold particles under optimised conditions. The polyclonal antibody appeared specific for the bovine antigen and immunocytological examination identified specific localisation of AIF in mitochondria, cytoplasm and nucleus of mammary epithelial cells in involuting mammary tissue, suggesting a subcellular translocation in cells undergoing apoptosis