We have developed a simple, inexpensive plate assay to detect and quantify polyphenol oxidase (PPO) activity from different origins. The logarithm of enzyme activity is linearly correlated with the diameter of the dark, L-3,4-dihydroxyphenylalanine (L-DOPA) oxidized circles produced in the gel, thereby allowing quantification of PPO. Moreover, precision and high reproducibility of the assay were confirmed by statistical analysis

3,4-Dihydroxyphenylalanine gel diffusion assay for polyphenol oxidase quantification

LOMOLINO, GIOVANNA;LANTE, ANNA
2008

Abstract

We have developed a simple, inexpensive plate assay to detect and quantify polyphenol oxidase (PPO) activity from different origins. The logarithm of enzyme activity is linearly correlated with the diameter of the dark, L-3,4-dihydroxyphenylalanine (L-DOPA) oxidized circles produced in the gel, thereby allowing quantification of PPO. Moreover, precision and high reproducibility of the assay were confirmed by statistical analysis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2469052
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