The results of two tests in vitro to evaluate antioxidant and antimicrobial activity of grape marc extract are presented. Bovine lymphocytes are incubated for 24 hours in presence of various extract concentrations (5 to 10.5 mg/mL) and subsequently stressed by addition of 15 μM (CuOH) hydroperoxide cumene solution. The intracellular production of ROS (reactive oxygen substances) are detected by partially modified method of DCF-DA (2,7 dichlorofluorescein diacetate), proposed by Rosenkranz et al. (1992). The antimicrobial activity test is carried out on agar plate inoculated by Bacillus stearothermophilus var. calidolactis C 953 spore. The appearance of a halo of inhibition after 24 hours of incubation confirmed the extract antimicrobial activity.
Test in vitro per Valutare l'attività antiossidante e antimicrobica di un estratto vegetale
LANTE, ANNA;LOMOLINO, GIOVANNA;GABAI, GIANFRANCO;
2007
Abstract
The results of two tests in vitro to evaluate antioxidant and antimicrobial activity of grape marc extract are presented. Bovine lymphocytes are incubated for 24 hours in presence of various extract concentrations (5 to 10.5 mg/mL) and subsequently stressed by addition of 15 μM (CuOH) hydroperoxide cumene solution. The intracellular production of ROS (reactive oxygen substances) are detected by partially modified method of DCF-DA (2,7 dichlorofluorescein diacetate), proposed by Rosenkranz et al. (1992). The antimicrobial activity test is carried out on agar plate inoculated by Bacillus stearothermophilus var. calidolactis C 953 spore. The appearance of a halo of inhibition after 24 hours of incubation confirmed the extract antimicrobial activity.Pubblicazioni consigliate
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